c facs buffer (Santa Cruz Biotechnology)
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C Facs Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+buffer+dapi/DAPI/us12240911-1323-8-22
Average 96 stars, based on 1456 article reviews
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FACS:Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules Article Snippet: Plates were finally resuspended in 90 μL/well FACS-buffer containing 0.2 μg/mL DAPI (Santa Cruz Biotec, Cat. No. Sc-3598) and acquired the same day using 5-laser LSR-FORTESSA (BD Bioscience with DIVA software). .. Plates were finally resuspended in 90 μL/well FACS-buffer containing 0.2 μg/mL Article Title: Bispecific antibodies specific for OX40 Article Snippet: .. Plates where washed twice with 200 μL/well 4° C. FACS buffer, were finally resuspended in 80 μL/well FACS-buffer containing 0.2 μg/mL Article Title: Bispecific antibodies specific for OX40 Article Snippet: Cells were stained with fluorescently labeled anti-human CD56 (clone NCAM16.2, mouse IgG1 κ, BioLegend, Cat.-No. 562751), anti-human CD25 (clone MA251, mouse IgG1 κ, BioLegend, Cat.-No. 356116) and anti-human CD69 (clone FN50, mouse IgG1 κ, BioLegend, Cat.-No. 356116) incubated for 20 minutes at 4° C. in the dark. .. Plates were finally resuspended in 80 μL/well FACS-buffer containing 0.2 μg/mL Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody Article Snippet: Cells were surface-stained with fluorescent dye-conjugated antibodies anti-human CD4 (clone RPA-T4, BioLegend, Cat.-No. 300532), CD8 (clone RPa-T8, BioLegend, Cat.-No. 3010441), CD62L (clone DREG-56, BioLegend, Cat.-No. 304834), CD127 (clone 019D5, BioLegend, Cat.-No. A019D5), CD134 (clone Ber-ACT35, BioLegend, Cat.-No. 350008), CD137 (clone 4B4-1, BioLegend, Cat.-No. 309814), GITR (clone 621, BioLegend, Cat.-No. 3311608) and CD25 (clone M-A251, BioLegend, Cat.-No. 356112) for 20 min at 4° C. in FACS buffer. .. Then, they were washed once with FACS buffer before being resuspended in 85 μL/well FACS buffer containing containing 0.2 μg/mL Software:Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules Article Snippet: Plates were finally resuspended in 90 μL/well FACS-buffer containing 0.2 μg/mL DAPI (Santa Cruz Biotec, Cat. No. Sc-3598) and acquired the same day using 5-laser LSR-FORTESSA (BD Bioscience with DIVA software). .. Plates were finally resuspended in 90 μL/well FACS-buffer containing 0.2 μg/mL Article Title: Bispecific antibodies specific for OX40 Article Snippet: .. Plates where washed twice with 200 μL/well 4° C. FACS buffer, were finally resuspended in 80 μL/well FACS-buffer containing 0.2 μg/mL Article Title: Bispecific antibodies specific for OX40 Article Snippet: Cells were stained with fluorescently labeled anti-human CD56 (clone NCAM16.2, mouse IgG1 κ, BioLegend, Cat.-No. 562751), anti-human CD25 (clone MA251, mouse IgG1 κ, BioLegend, Cat.-No. 356116) and anti-human CD69 (clone FN50, mouse IgG1 κ, BioLegend, Cat.-No. 356116) incubated for 20 minutes at 4° C. in the dark. .. Plates were finally resuspended in 80 μL/well FACS-buffer containing 0.2 μg/mL Article Title: Combination therapy with targeted 4-1BB (CD137) agonists/anti-FAP binding domain and anti-CEA/anti-CD3 bispecific antibody Article Snippet: Cells were surface-stained with fluorescent dye-conjugated antibodies anti-human CD4 (clone RPA-T4, BioLegend, Cat.-No. 300532), CD8 (clone RPa-T8, BioLegend, Cat.-No. 3010441), CD62L (clone DREG-56, BioLegend, Cat.-No. 304834), CD127 (clone 019D5, BioLegend, Cat.-No. A019D5), CD134 (clone Ber-ACT35, BioLegend, Cat.-No. 350008), CD137 (clone 4B4-1, BioLegend, Cat.-No. 309814), GITR (clone 621, BioLegend, Cat.-No. 3311608) and CD25 (clone M-A251, BioLegend, Cat.-No. 356112) for 20 min at 4° C. in FACS buffer. .. Then, they were washed once with FACS buffer before being resuspended in 85 μL/well FACS buffer containing containing 0.2 μg/mL other:Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules Article Snippet: NF-κB Activation in HeLa Cells Expressing Human 4-1BB Generation of HeLa Cells Expressing Human 4-1BB and NF-κB-Luciferase The cervix carcinoma cell line HeLa (ATCC CCL-2) was transduced with a plasmid based on the expression vector pETR10829, which contains the sequence of human 4-1BB (Uniprot accession Q07011) under control of a CMV-promoter and a puromycin resistance gene. Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules Article Snippet: If cells were stained with fixable viability dyes, they were fixed with 50 μL/well DPBS containing 1% formaldehyde (Sigma, HT501320-9.5L). |
